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3, 3′, 5-triiodo-L-thyronine increases in vitro chondrogenesis of mesenchymal stem cells from human umbilical cord stroma through SRC2

Identificadores del recurso

Fernández-Pernas P, Fafián-Labora J, Lesende-Rodriguez I, Mateos J, De la Fuente A, Fuentes I, De Toro Santos J, Blanco García F, Arufe MC. 3, 3', 5-triiodo-L-thyronine Increases In Vitro Chondrogenesis of Mesenchymal Stem Cells From Human Umbilical Cord Stroma Through SRC2. J Cell Biochem. 2016 Sep;117(9):2097-108.

0730-2312

1097-4644

http://hdl.handle.net/2183/18332

Procedencia

(RUC. Repositorio da Universidade da Coruña)

Ficha

Título:: 3, 3′, 5-triiodo-L-thyronine increases in vitro chondrogenesis of mesenchymal stem cells from human umbilical cord stroma through SRC2
Tema:: Mesenchymal stem cells; Human umbilical cord stroma; Chondrocyte-like cells; Chondrogenesis; Prolactin; 3, 3', 5-triodo-L-thyronine
Descrición:: [Abstract] Our group focuses on the study of mesenchymal stem cells (MSCs) from human umbilical cord stroma or Warthońs jelly and their directed differentiation toward chondrocyte-like cells capable of regenerating damaged cartilage when transplanted into an injured joint. This study aimed to determine whether lactogenic hormone prolactin (PRL) or 3, 3′, 5-triiodo-L-thyronine (T3), the active thyroid hormone, modulates chondrogenesis in our in vitro model of directed chondrogenic differentiation, and whether Wnt signalling is involved in this modulation. MSCs from human umbilical cord stroma underwent directed differentiation toward chondrocyte-like cells by spheroid formation. The addition of T3 to the chondrogenic medium increased the expression of genes linked to chondrogenesis like collagen type 2, integrin alpha 10 beta 1, and Sox9 measured by quantitative real time polymerase chain reaction (qRT-PCR) analysis. Levels of collagen type 2 and aggrecane analyzed by immunohistochemistry, and staining by Safranin O were increased after 14 days in spheroid culture with T3 compared to those without T3 or only with PRL. B-catenin, Frizzled, and GSK-3β gene expressions were significantly higher in spheroids cultured with chondrogenic medium (CM) plus T3 compared to CM alone after 14 days in culture. The increase of chondrogenic differentiation was inhibited when the cells were treated with T3 plus ML151, an inhibitor of the T3 steroid receptor. This work demonstrates, for first time, that T3 promotes differentiation towards chondrocytes-like cells in our in vitro model, that this differentiation is mediated by steroid receptor co-activator 2 (SRC2) and does not induce hypertrophy.; This study was supported by a grant from Instituto de Salud Carlos III—Ministerio Economía y Competitividad N° Expediente PI11/02799 Unión Europea—Fondo Europeo de Desarrollo Regional (FEDER) “Una manera de hacer Europa".; info:eu-repo/grantAgreement/MICINN/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/PI11%2F02799/ES/Combinación de terapias celulares para la reparación de lesiones de cartílago
Idioma:: English
Relación:: http://dx.doi.org/10.1002/jcb.25515
Autor/Produtor:: Fernández-Pernas, Pablo; Fafián Labora, Juan Antonio; Lesende-Rodríguez, Iván; Mateos, Jesús; Fuente, Alexandre de la; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Arufe, M.C.
Editor:: Wiley
Dereitos:: This is the peeer reviewed version of the article which has been published in final form at Wiley Online Library. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving; info:eu-repo/semantics/embargoedAccess
Data:: 2016-07-08
Tipo de recurso:: info:eu-repo/semantics/article

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2. < dc:creator > Fernández-Pernas, Pablo </ dc:creator >
3. < dc:creator > Fafián Labora, Juan Antonio </ dc:creator >
4. < dc:creator > Lesende-Rodríguez, Iván </ dc:creator >
5. < dc:creator > Mateos, Jesús </ dc:creator >
6. < dc:creator > Fuente, Alexandre de la </ dc:creator >
7. < dc:creator > Fuentes Boquete, Isaac Manuel </ dc:creator >
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1. < dc:title > 3, 3′, 5-triiodo-L-thyronine increases in vitro chondrogenesis of mesenchymal stem cells from human umbilical cord stroma through SRC2 </ dc:title >
2. < dc:creator > Fernández-Pernas, Pablo </ dc:creator >
3. < dc:creator > Fafián Labora, Juan Antonio </ dc:creator >
4. < dc:creator > Lesende-Rodríguez, Iván </ dc:creator >
5. < dc:creator > Mateos, Jesús </ dc:creator >
6. < dc:creator > Fuente, Alexandre de la </ dc:creator >
7. < dc:creator > Fuentes Boquete, Isaac Manuel </ dc:creator >
8. < dc:creator > De-Toro, Javier </ dc:creator >
9. < dc:creator > Blanco García, Francisco J </ dc:creator >
10. < dc:creator > Arufe, M.C. </ dc:creator >
11. < dc:subject > Mesenchymal stem cells </ dc:subject >
12. < dc:subject > Human umbilical cord stroma </ dc:subject >
13. < dc:subject > Chondrocyte-like cells </ dc:subject >
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15. < dc:subject > Prolactin </ dc:subject >
16. < dc:subject > 3, 3', 5-triodo-L-thyronine </ dc:subject >
17. < dcterms:abstract > [Abstract] Our group focuses on the study of mesenchymal stem cells (MSCs) from human umbilical cord stroma or Warthońs jelly and their directed differentiation toward chondrocyte-like cells capable of regenerating damaged cartilage when transplanted into an injured joint. This study aimed to determine whether lactogenic hormone prolactin (PRL) or 3, 3′, 5-triiodo-L-thyronine (T3), the active thyroid hormone, modulates chondrogenesis in our in vitro model of directed chondrogenic differentiation, and whether Wnt signalling is involved in this modulation. MSCs from human umbilical cord stroma underwent directed differentiation toward chondrocyte-like cells by spheroid formation. The addition of T3 to the chondrogenic medium increased the expression of genes linked to chondrogenesis like collagen type 2, integrin alpha 10 beta 1, and Sox9 measured by quantitative real time polymerase chain reaction (qRT-PCR) analysis. Levels of collagen type 2 and aggrecane analyzed by immunohistochemistry, and staining by Safranin O were increased after 14 days in spheroid culture with T3 compared to those without T3 or only with PRL. B-catenin, Frizzled, and GSK-3β gene expressions were significantly higher in spheroids cultured with chondrogenic medium (CM) plus T3 compared to CM alone after 14 days in culture. The increase of chondrogenic differentiation was inhibited when the cells were treated with T3 plus ML151, an inhibitor of the T3 steroid receptor. This work demonstrates, for first time, that T3 promotes differentiation towards chondrocytes-like cells in our in vitro model, that this differentiation is mediated by steroid receptor co-activator 2 (SRC2) and does not induce hypertrophy. </ dcterms:abstract >
18. < dcterms:issued > 2016-07-08 </ dcterms:issued >
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23. < dc:identifier > http://hdl.handle.net/2183/18332 </ dc:identifier >
24. < dc:language > eng </ dc:language >
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  1. < dc:title > 3, 3′, 5-triiodo-L-thyronine increases in vitro chondrogenesis of mesenchymal stem cells from human umbilical cord stroma through SRC2 </ dc:title >
  2. < dc:creator > Fernández-Pernas, Pablo </ dc:creator >
  3. < dc:creator > Fafián Labora, Juan Antonio </ dc:creator >
  4. < dc:creator > Lesende-Rodríguez, Iván </ dc:creator >
  5. < dc:creator > Mateos, Jesús </ dc:creator >
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  9. < dc:creator > Blanco García, Francisco J </ dc:creator >
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  11. < dc:subject > Mesenchymal stem cells </ dc:subject >
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  13. < dc:subject > Chondrocyte-like cells </ dc:subject >
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  17. < dc:description > [Abstract] Our group focuses on the study of mesenchymal stem cells (MSCs) from human umbilical cord stroma or Warthońs jelly and their directed differentiation toward chondrocyte-like cells capable of regenerating damaged cartilage when transplanted into an injured joint. This study aimed to determine whether lactogenic hormone prolactin (PRL) or 3, 3′, 5-triiodo-L-thyronine (T3), the active thyroid hormone, modulates chondrogenesis in our in vitro model of directed chondrogenic differentiation, and whether Wnt signalling is involved in this modulation. MSCs from human umbilical cord stroma underwent directed differentiation toward chondrocyte-like cells by spheroid formation. The addition of T3 to the chondrogenic medium increased the expression of genes linked to chondrogenesis like collagen type 2, integrin alpha 10 beta 1, and Sox9 measured by quantitative real time polymerase chain reaction (qRT-PCR) analysis. Levels of collagen type 2 and aggrecane analyzed by immunohistochemistry, and staining by Safranin O were increased after 14 days in spheroid culture with T3 compared to those without T3 or only with PRL. B-catenin, Frizzled, and GSK-3β gene expressions were significantly higher in spheroids cultured with chondrogenic medium (CM) plus T3 compared to CM alone after 14 days in culture. The increase of chondrogenic differentiation was inhibited when the cells were treated with T3 plus ML151, an inhibitor of the T3 steroid receptor. This work demonstrates, for first time, that T3 promotes differentiation towards chondrocytes-like cells in our in vitro model, that this differentiation is mediated by steroid receptor co-activator 2 (SRC2) and does not induce hypertrophy. </ dc:description >
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  20. < dc:date > 2017-07-16 </ dc:date >
  21. < dc:date > 2017-07-16 </ dc:date >
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